In vitro UV mutagenesis associated with nucleotide excision-repair gaps in Escherichia coli.
نویسندگان
چکیده
منابع مشابه
The Role of Nucleotide Excision Repair in Restoring Replication Following UV-Induced Damage in Escherichia coli
Following low levels of UV exposure, Escherichia coli cells deficient in nucleotide excision repair (uvrA6 mutants) recover and synthesize DNA at near wild type levels, an observation that formed the basis of the post-replication recombination repair model. In this study, we characterized the DNA synthesis that occurs following UV-irradiation in the absence of nucleotide excision repair and sho...
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Following the irradiation of nondividing yeast cells with ultraviolet (UV) light, most induced mutations are inherited by both daughter cells, indicating that complementary changes are introduced into both strands of duplex DNA prior to replication. Early analyses demonstrated that such two-strand mutations depend on functional nucleotide excision repair (NER), but the molecular mechanism of th...
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One form of nucleotide excision repair (NER) is known to be functionally coupled to transcription, but the nature of this functional link in Escherichia coli is still unclear. Here we have employed the isolated membrane-associated nucleoids from E.coli to examine this issue. We show that the isolated nucleoid fraction is capable of excision of UV-induced pyrimidine dimers when reconstituted wit...
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Nucleotide excision repair and translesion DNA synthesis are two processes that operate at arrested replication forks to reduce the frequency of recombination and promote cell survival following UV-induced DNA damage. While nucleotide excision repair is generally considered to be error free, translesion synthesis can result in mutations, making it important to identify the order and conditions ...
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Multiple mutant strains have been tested or their mimicry of the UV-mutagenesis deficiency of a recA single mutant. Revertants to histidine prototrophy and clear plaque mutants of lambda were scored to determine capacity for UV-mutagenesis. Nearly normal capacity was shown by a uvr+ recB- recF- strain, which shows almost no recA-dependent recombination, by uvr- recB+ recF- strains, which show a...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)37638-x